Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Development and epigenetic plasticity of murine Müller glia

doi: 10.1016/j.bbamcr.2019.06.019

Figure Lengend Snippet: List of oligonucleotides and antibodies used in this study

Article Snippet: Isolation of Notch1+ cells, Glast+ cells, and cortical astrocytes We used the same protocol for immunomagnetic cell separation described in Dvoriantchikova et al. to isolate Notch1+ cells with monoclonal biotin-conjugated anti-Notch1 mouse antibodies (130–096-557, Miltenyi Biotec, Auburn, CA) [ 18 ].

Techniques:

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Development and epigenetic plasticity of murine Müller glia

doi: 10.1016/j.bbamcr.2019.06.019

Figure Lengend Snippet: Quantitative RT-PCR analysis has confirmed the microarray data for a group of selected genes. For each gene, the results are expressed as percentages ± SEM of the corresponding values in the Notch1+ cells isolated from P0 developing retinas (P0 Notch1+ cells).

Article Snippet: Isolation of Notch1+ cells, Glast+ cells, and cortical astrocytes We used the same protocol for immunomagnetic cell separation described in Dvoriantchikova et al. to isolate Notch1+ cells with monoclonal biotin-conjugated anti-Notch1 mouse antibodies (130–096-557, Miltenyi Biotec, Auburn, CA) [ 18 ].

Techniques: Quantitative RT-PCR, Microarray, Isolation

Journal: Biochimica et biophysica acta. Molecular cell research

Article Title: Development and epigenetic plasticity of murine Müller glia

doi: 10.1016/j.bbamcr.2019.06.019

Figure Lengend Snippet: The results of the hierarchical and k-means clustering revealed the changes that are undergone in RPCs (Notch1+ cells) and Müller glia (Glast+ cells) during retinal development. A) Hierarchical clustering of differentially expressed genes showed that many P7 and P14 Notch1+ cells (late RPCs) are predisposed to become Müller glia. While Glast+ cells at P7 are still very close to a multipotent progenitor state, P14, P21, and P28 Glast+ cells are already grouped together indicating the adult Müller glia state. B) The 10 clusters were identified by the k-means clustering algorithm. C) The majority of differentially expressed genes were located in cluster 1, 3, and 4. D) Meanwhile, significant changes were undergone in genes located in small clusters 5, 7, and 8. A value F (F statistic) was calculated using the ANOVA test.

Article Snippet: Isolation of Notch1+ cells, Glast+ cells, and cortical astrocytes We used the same protocol for immunomagnetic cell separation described in Dvoriantchikova et al. to isolate Notch1+ cells with monoclonal biotin-conjugated anti-Notch1 mouse antibodies (130–096-557, Miltenyi Biotec, Auburn, CA) [ 18 ].

Techniques: